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panck  (Novus Biologicals)


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    Structured Review

    Novus Biologicals panck
    Panck, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pan+cytokeratin/Cytokeratin%2C+pan+Antibody+(AE-1%2FAE-3)+-+Azide+and+BSA+Free/pm41986711-543-15-20
    Average 95 stars, based on 47 article reviews
    panck - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Immune infiltration is linked to increased transcriptional activity and distinct metabolic profiles in chordomas
    Article Snippet: In brief, slides were fixed using 10% neutral-buffered formalin, followed by overnight in situ hybridization using the Human NGS Whole Transcriptome Atlas (WTA) RNA (v1.0) probe set (NanoString Technologies). .. Slides were concurrently incubated with immunofluorescent antibodies, including pan-cytokeratin (AE-1/AE3, Novus Biologicals) to identify tumor cells and CD45 (2B11 + PD7/26, Novus Biologicals) to detect immune cells. .. Next, slides were stained with the GeoMx Nuclear Stain (Syto13, Thermo Fisher Scientific), after which slides were loaded into the GeoMx Digital Spatial Profiler instrument (NanoString Technologies) and scanned.

    Article Title: Tumor-Initiating Stem Cells Fine-tune the Plasticity of Neutrophils to Sculpt a Protective Niche
    Article Snippet: Antigen retrieval was performed using a low-pH antigen retrieval buffer (Thermo Fisher Scientific). .. Slides were then blocked at room temperature for 1 hour and incubated overnight at 4 °C in a humidified chamber with the following antibodies: pan cytokeratin (rabbit, 1:100, Abcam), pan cytokeratin (mouse, 1:200, Novus Biologicals), SOX2 (rat, 1:100, Thermo Fisher Scientific), MPO (goat, 1:40, R&D Systems), CD4 (rabbit, 1:500, Abcam), or HLA-DR (mouse, 1:500, Thermo Fisher Scientific). .. After primary antibody incubation, slides were washed three times with PBS, each for 5 minutes, and then incubated with secondary antibodies conjugated with Alexa Fluor 488, Rhodamine Red, or Alexa Fluor 647 (Jackson ImmunoResearch Laboratories).

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer
    Article Snippet: .. Formally fixed paraffin-embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan-cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer.
    Article Snippet: .. Formally fixed paraffin- embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan- cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Article Title: Tumor-initiating stem cells fine-tune the plasticity of neutrophils to sculpt a protective niche.
    Article Snippet: Immunofluorescence staining of FFPE slides of human HNSCC samples FFPE slides were deparaffinized by incubation at 60◦C for 1 h. Rehydration was performed through sequential washes: three times in xylene, followed by 100% ethanol, 95% ethanol, 75% ethanol, and distilled water (ddH2O), each for 10 min. Antigen retrieval was performed using a low-pH antigen retrieval buffer (Thermo Fisher Scientific). .. Slides were then blocked at room temperature for 1 h and incubated overnight at 4◦C in a humidified chamber with the following antibodies: pan cytokeratin (rabbit, 1:100, Abcam), pan cytokeratin (mouse, 1:200, Novus Biologicals), SOX2 (rat, 1:100, Thermo Fisher Scientific), MPO (goat, 1:40, R&D Systems), CD4 (rabbit, 1:500, Abcam), or HLA-DR (mouse, 1:500, Thermo Fisher Scientific). .. After primary antibody incubation, slides were washed three times with PBS, each for 5 min, and then incubated with secondary antibodies conjugated with Alexa Fluor 488, Rhodamine Red, or Alexa Fluor 647 (Jackson ImmunoResearch Laboratories).

    Article Title: Integrated analysis of stemness-associated immune modulatory circuits in squamous cell carcinomas
    Article Snippet: Antigen retrieval was performed using a low-pH antigen retrieval buffer (Thermo Fisher Scientific). .. Slides were then blocked at room temperature for 1 hour and incubated overnight at 4°C in a humidified chamber with the following antibodies: pan cytokeratin (rabbit, 1:100, Abcam, Cat# ab234297; RRID: AB_2895302), pan cytokeratin (mouse, 1:200, Novus Biologicals, Cat# NBP2-29429; RRID: AB_3068002), SOX2 (rat, 1:100, Thermo Fisher Scientific, Cat# 14-9811-82; RRID: AB_11219471), MPO (goat, 1:40, R&D Systems, Cat# AF3667; RRID: AB_2250866),. .. After primary antibody incubation, slides were washed three times with PBS, each for 5 minutes, and then incubated with secondary antibodies conjugated with Alexa Fluor 488, Rhodamine Red, or Alexa Fluor 647 (Jackson ImmunoResearch Laboratories).

    Staining:

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer
    Article Snippet: .. Formally fixed paraffin-embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan-cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer.
    Article Snippet: .. Formally fixed paraffin- embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan- cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Article Title: High‐Plex Digital Spatial Profiling Identified Prolactin‐Induced Protein mRNA Associated With Response and Survival of Everolimus and Letrozole Treatment for Hormone Receptor‐Positive/Human Epidermal Growth Factor Receptor 2‐Negative Advanced Breast Cancer
    Article Snippet: The GeoMx Morphology Kit for RNA (NanoString, USA) was used for tissue visualization to guide the selection of ROI. .. The antibodies used as morphology markers were Pan‐cytokeratin (PANCK, Novus NEP2–33200) for tumor and epithelial cells, CD45 ( CST13917 ) for leucocytes, plus a nuclear staining SYTO13 (NanoString 121300310). ..

    Clone Assay:

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer
    Article Snippet: .. Formally fixed paraffin-embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan-cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Article Title: Genetically engineered ErbB2 overexpression sensitizes organoid-derived tumors to checkpoint inhibition in a syngeneic model of gastric cancer.
    Article Snippet: .. Formally fixed paraffin- embedded longitudinal sections of stomach or subcutaneous tumor tissue were stained with the following primary antibody clones or polyclonal sera (supplier, dilution, and incubation time are given): CD3 Clone SP7 (Abcam, 1:250, o/n), pan- cytokeratin clone PCK26 (Novus Biologicals, 1:1500, 1 hour) followed by detection with EnVision secondary reagents and DAB. ..

    Labeling:

    Article Title: Biomechanical Phenotyping Reveals Unique Mechanobiological Signatures of Early‐Onset Colorectal Cancer
    Article Snippet: Fresh cuts of 5 μm FFPE sections were mounted on charged slides, baked, and stained using the BOND RX/RXm Fully Automated IHC/ISH Stainer from Leica Biosystems, following the manufacturer's automated slide preparation user manual. .. IF labeling of distinct cell compartments was conducted using the following markers: epithelial cells via pan‐cytokeratin (PanCK, Novus NBP2‐33200AF532‐AF532; 1:40); non‐immune stromal cells via Vimentin (Santa Cruz sc373717‐AF594, 1:150); leukocytes via CD45 (Nanostring CD45‐AF647, clone D9M8I, CST cat number 13 917; 5 μg mL −1 ), cell nuclei via SYTO13 (Nanostring; 1:10). ..



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    Image Search Results


    Representative microscopic images of Cytokeratin (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.

    Journal: International Journal of Molecular Sciences

    Article Title: Disseminated Tumor Cells (DTCs) in Patients with Cervical Cancer Reveal Mesenchymal Properties and Potential Therapeutic Targets—A New Perspective?

    doi: 10.3390/ijms27114875

    Figure Lengend Snippet: Representative microscopic images of Cytokeratin (CK) positive tumor cells. ( A ) Reference Cell (HCT116) from a control slide. ( B ) Patient-derived circulating tumor cell (CTC) which stained positive for CK and ( C ) disseminated tumor cell (DTC) from the same patient that stainend positive against CK. CK-positive cells are visualized in pink, while nuclei are counterstained in blue. Images were processed and annotated using Aperio ImageScope software Version 12.4.6 (Leica Biosystems, Nußloch, Germany) and the figure was assembled using Microsoft PowerPoint.

    Article Snippet: In the first round of staining, anti-human Pan-Cytokeratin APC-conjugated REAdye_lease antibody (Cat. No. 130-123-091, Miltenyi Biotech, Bergisch Gladbach, Germany), anti-human vimentin FITC-conjugated REAdye_lease antibody (Cat. No. 130-127-022, Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-human VEGF PE-conjugated REAdye_lease antibody (Cat. No. 130-118-061, Miltenyi Biotech, Bergisch Gladbach, Germany) were applied.

    Techniques: Control, Derivative Assay, Staining, Software

    Positive and negative control cells for both marker panels. ( A ) HeLa (white arrows): in the first panel positive for cytokeratin (CK), Vimentin (Vim), VEGF and in the second panel positive for p16INK4A, negative for PD-L1 and CD45. ( B ) T98G (red arrows): positive for Vim. ( C ) CaSki (yellow arrows): positive for CK, Vim, VEGF, PD-L1 and p16INK4A. ( D ) MCF-7 (green arrows): positive for CK. All cell lines were negative for CD45. Only hematopoietic cells showed CD45 positive staining. Scale bar = 10 μm. Images were processed using Zeiss ZEN 3.7 software and the figure was assembled using Microsoft PowerPoint.

    Journal: International Journal of Molecular Sciences

    Article Title: Disseminated Tumor Cells (DTCs) in Patients with Cervical Cancer Reveal Mesenchymal Properties and Potential Therapeutic Targets—A New Perspective?

    doi: 10.3390/ijms27114875

    Figure Lengend Snippet: Positive and negative control cells for both marker panels. ( A ) HeLa (white arrows): in the first panel positive for cytokeratin (CK), Vimentin (Vim), VEGF and in the second panel positive for p16INK4A, negative for PD-L1 and CD45. ( B ) T98G (red arrows): positive for Vim. ( C ) CaSki (yellow arrows): positive for CK, Vim, VEGF, PD-L1 and p16INK4A. ( D ) MCF-7 (green arrows): positive for CK. All cell lines were negative for CD45. Only hematopoietic cells showed CD45 positive staining. Scale bar = 10 μm. Images were processed using Zeiss ZEN 3.7 software and the figure was assembled using Microsoft PowerPoint.

    Article Snippet: In the first round of staining, anti-human Pan-Cytokeratin APC-conjugated REAdye_lease antibody (Cat. No. 130-123-091, Miltenyi Biotech, Bergisch Gladbach, Germany), anti-human vimentin FITC-conjugated REAdye_lease antibody (Cat. No. 130-127-022, Miltenyi Biotech, Bergisch Gladbach, Germany) and anti-human VEGF PE-conjugated REAdye_lease antibody (Cat. No. 130-118-061, Miltenyi Biotech, Bergisch Gladbach, Germany) were applied.

    Techniques: Negative Control, Marker, Staining, Software